Serum Biomarker for Diagnosis of Endometriosis

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Abstract

Endometriosis is estimated to affect 10% of women during the reproductive years. The lack of a non-invasive diagnostic test significantly contributes to the long delay between onset of the symptoms and definitive diagnosis of endometriosis. This case-control study was conducted to identify specific endometriosis antigens using 2D gel analysis in women with endometriosis (n = 5) and without endometriosis (n = 5). Differentially expresses spots were analyzed using matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (nanoLC-ESI-MS/MS) with MASCOT analysis, in order to identify the corresponding proteins. ELISAs were performed on a different cohort of endometriosis (n = 120) and healthy patients (n = 20) in order to confirm the differential expression of the identified proteins. ROC analysis of ELISA results confirmed the statistical significance of the differential expression for one of these proteins: Zn-alpha2-glycoprotein (P = 0.019). We propose the analysis of the expression level of this protein in the serum as a new non-invasive diagnostic test for endometriosis.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Biomarkers Endometriosis Endometriosis Adult Biomarkers Biomarkers Case-Control Studies Electrophoresis, Gel, Two-Dimensional Endometriosis Female Humans Mass Spectrometry Proteins Proteins Proteins Reproducibility of Results ROC Curve Silver Staining

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