Prototype Salivary Assay for Quantification of Two Biomarkers for In Vitro Diagnosis of Endometriosis

Critical Reviews™ in Eukaryotic Gene Expression Published 8 issues per year ISSN Print: 1045-4403 ISSN Online: 2162-6502 Research Article Prototype Salivary Assay for Quantification of Two Biomarkers for In Vitro Diagnosis of Endometriosis ABSTRACT Endometriosis, a very common disease in women, is characterized by endometrial structures outside the uterine cavity. The lack of a reliable noninvasive diagnostic test and the often nonspecific symptoms of this pathology are responsible for the delay in definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we describe the experimental conditions of a simple ELISA for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. 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