Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis

Gazvani R, Rafet Gazvani, Louise Smith, Smith L, Paul A Fowler, Paul Fowler
Fertility and sterility · 2002 · vol. 77(1) , pp. 62–67 · doi:10.1016/s0015-0282(01)02954-5 · PMID:11779592 · W2171761307
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This study investigated the effects of IL-8, anti-IL-8, and IL-12 on the survival of endometrial gland and stromal cells cultured from women with and without endometriosis.

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Abstract

OBJECTIVE: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells. DESIGN: An in vitro study. SETTING: Department of Obstetrics and Gynecology, University of Aberdeen, UK. PATIENT(S): Women attending a fertility clinic. INTERVENTION(S): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 microg/mL, 1 microg/mL, 10 microg/mL). MAIN OUTCOME MEASURE(S): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture. RESULT(S): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis, IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 microg/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture. CONCLUSION(S): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometrium Endometrium Interleukin-12 Interleukin-8 Cell Culture Techniques Cell Culture Techniques Cell Culture Techniques Cells, Cultured Cells, Cultured Dose-Response Relationship, Drug Dose-Response Relationship, Drug Endometriosis Endometriosis Endometrium Endometrium Endometrium Female Female Humans

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