Combination of CCR1 mRNA, MCP1, and CA125 Measurements in Peripheral Blood as a Diagnostic Test for Endometriosis

Admir Agic, Schima Djalali, Monika M Wolfler, Monika Wölfler, Gulden Halis, Gülden Halis, Klaus Diedrich, Daniela Hornung
Reproductive sciences (Thousand Oaks, Calif.) · 2008 · vol. 15(9) , pp. 906–911 · doi:10.1177/1933719108318598 · PMID:19050323 · W1991956657
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Measuring CCR1 mRNA in leukocytes combined with serum MCP-1 and CA125 levels demonstrated high sensitivity and specificity for diagnosing endometriosis.

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The paper evaluated whether measuring CCR1 mRNA in peripheral blood leukocytes, combined with serum monocyte chemotactic protein-1 (MCP-1) and CA125, could serve as a non-surgical diagnostic test for endometriosis. CCR1/HPRT mRNA ratios were quantified by quantitative real-time PCR, while MCP-1 and CA125 were measured in serum by ELISA and ECLIA; compared with women without endometriosis, patients with endometriosis and adenomyosis showed significantly elevated CCR1/HPRT and higher serum MCP-1 and CA125 levels. The reported diagnostic performance included sensitivity of 92.2% and specificity of 81.6%, with corresponding predictive values and likelihood ratios described in the abstract. This paper is centrally about endometriosis — specifically a combined peripheral blood CCR1 mRNA, MCP-1, and CA125 biomarker approach to diagnose or exclude endometriosis.

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Abstract

This study investigated the possible use of CCR1 mRNA measurement in peripheral blood leukocytes in combination with measurements of monocyte chemotactic protein-1 (MCP-1) and CA125 protein in serum as a diagnostic test for endometriosis. The expression of CCR1 mRNA in peripheral blood leukocytes was measured by quantitative real-time polymerase chain reaction. MCP-1 and CA125 levels in serum were determined by ELISA and ECLIA.The ratio of CCR1/HPRT mRNA in peripheral blood of patients with endometriosis and adenomyosis was significantly elevated compared with women without endometriosis. Additionally, serum levels of MCP-1 and CA125 were significantly higher in patients with endometriosis. This method showed a sensitivity of 92.2%, a specificity of 81.6%, a negative predictive value of 83.3%, a positive predictive value of 92.3%, a likelihood ratio of a positive test result of 5.017, and a likelihood ratio of a negative test result of 0.096 to predict the presence or absence of endometriosis. The results imply the potential use of CCR1 mRNA, MCP-1, and CA125 protein measurements for the diagnosis or exclusion of endometriosis. Similar content being viewed by others

References

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Husby GK, Haugen RS, Moen MH. Diagnostic delay in women with pain and endometriosis. Acta Obstet Gynecol Scand. 2003;82:649–653. Author information Authors and Affiliations Corresponding author Rights and permissions About this article Cite this article Agic, A., Djalali, S., Wolfler, M.M. et al. Combination of CCR1 mRNA, MCP1, and CA125 Measurements in Peripheral Blood as a Diagnostic Test for Endometriosis. Reprod. Sci. 15, 906–911 (2008). https://doi.org/10.1177/1933719108318598 Published: Issue date: DOI: https://doi.org/10.1177/1933719108318598

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mesh:D004715endometriosis

MeSH descriptors

CA-125 Antigen Chemokine CCL2 Endometriosis Receptors, CCR1 CA-125 Antigen Chemokine CCL2 Endometriosis Endometriosis Female Humans Receptors, CCR1 Receptors, CCR1 Retrospective Studies RNA, Messenger RNA, Messenger

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