Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis

R Cosín, J Gilabert-Estellés, Juan Gilabert–Estellés, L A Ramón, Luis Ramón, M J Gómez-Lechón, J Gilabert, M Chirivella, Aitana Braza‐Boïls, A Braza-Boïls, Francisco España, F España, A Estellés
Human Reproduction · 2009 · vol. 25(2) , pp. 398–405 · doi:10.1093/humrep/dep419 · PMID:19945964 · W2169524641
article OA: bronze CC0 ⤵ 36 in-corpus citations
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Endometriotic peritoneal fluid increased VEGF-A and uPA expression in endometrial cells from women with and without endometriosis, with the most significant increase observed in cells from endometriosis patients.

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Abstract

BACKGROUND: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent benign gynaecological diseases. It has been suggested that both endometrial and peritoneal factors, related to angiogenesis and proteolysis, can be implicated in this disease. The aim of this study was to evaluate the influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with and without endometriosis. METHODS: Endometrial cells were isolated, cultured and treated with endometriotic or normal peritoneal fluid. Vascular endothelial growth factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels were evaluated by quantitative RT-PCR, and protein levels were quantified by ELISA. RESULTS: Peritoneal fluid from women with endometriosis induced an increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in endometrial cell culture from women with (P < 0.01) and without endometriosis (P < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell cultures from patients with endometriosis and treated with peritoneal fluid from women with endometriosis. CONCLUSIONS: Peritoneal fluid from women with endometriosis induced more VEGF and uPA expression in endometrial cell culture from women with endometriosis than did normal peritoneal fluid. Endometrial-peritoneal interactions increased angiogenic and proteolytic factors in endometrial cells, which could contribute to the development of endometriotic lesions.

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Condition tags

mesh:D004715endometriosis

MeSH descriptors

Angiogenic Proteins Ascitic Fluid Endometriosis Endometrium Peptide Hydrolases Adult Angiogenic Proteins Ascitic Fluid Cells, Cultured Endometriosis Endometrium Female Humans Matrix Metalloproteinase 3 Matrix Metalloproteinase 3 Peptide Hydrolases Plasminogen Activator Inhibitor 1 Plasminogen Activator Inhibitor 1 Thrombospondin 1 Thrombospondin 1

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