C/EBPδ regulates SPHK1-dependent excessive autophagy in decidualization defects of chronic endometritis

Jiahui Xiao, Shuyi Yu, Shiru Xu, Guanying You, Meilan Mo, Ruochun Lian, Cong Chen, Xueling Zhang, Yuye Li
Reproduction (Cambridge, England) · 2026 · vol. 172(3) · doi:10.1093/reprod/xaag111 · PMID:42704675 · W7211904965
Lab / animal OA: closed CC0
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Chronic endometritis impairs decidualization by suppressing the C/EBPδ–SphK1 axis, which reduces S1P levels and causes excessive autophagy via S1PR3 signaling.

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Abstract

In brief: Chronic endometritis (CE) impairs endometrial receptivity via unknown mechanisms. This study reveals that suppression of the C/EBPδ-sphingosine kinase 1 (SphK1)-sphingosine-1-phosphate (S1P) axis in CE leads to decidualization defects accompanied by excessive autophagy, providing a mechanistic link between chronic inflammation, sphingolipid dysregulation, and impaired endometrial receptivity. Abstract: Chronic endometritis (CE) is a persistent inflammatory disorder of the endometrium that is increasingly recognized as an important cause of reproductive failure. However, the molecular mechanisms by which CE impairs endometrial receptivity remain incompletely understood. Here, we found that decidualization markers IGFBP1 and PRL were significantly decreased in CE patients, accompanied by abnormal glandular architecture. Transcriptomic analysis revealed sphingolipid metabolism as a dysregulated pathway, with sphingosine kinase 1 (SphK1) markedly downregulated. Sphingosine-1-phosphate (S1P) levels were reduced in both CE tissues and lipopolysaccharide-treated stromal cells during decidualization. Functional experiments confirmed that SphK1 is essential for this process, and its deficiency impairs decidualization. Mechanistically, transcription factor C/EBPδ directly bound and activated the SphK1 promoter. Chronic endometritis endometrium showed reduced C/EBPδ and SphK1, alongside increased LC3B, indicating autophagic dysregulation. Furthermore, S1P was demonstrated to act extracellularly through the G protein-coupled receptor S1P receptor 3 (S1PR3) to maintain autophagic homeostasis, thereby regulating endometrial decidualization. In conclusion, suppression of the C/EBPδ-SphK1 axis impairs decidualization with excessive autophagy, revealing a mechanistic link between chronic inflammation, sphingolipid dysregulation, and reproductive failure.

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MeSH descriptors

Autophagy CCAAT-Enhancer-Binding Proteins CCAAT-Enhancer-Binding Proteins Decidua Decidua Decidua Endometritis Endometritis Endometritis Endometritis Phosphotransferases (Alcohol Group Acceptor) Phosphotransferases (Alcohol Group Acceptor) Phosphotransferases (Alcohol Group Acceptor) Animals Chronic Disease Endometrium Endometrium Endometrium Female Humans

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References (35)

SciLite annotations

chemicals 2
sphingolipid sphingolipid

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License: CC0 · commercial use OK