Transcriptome analysis of eutopic endometrial stromal cells in women with adenomyosis by RNA-sequencing

Lin Gan, Yongrong Li, Yan Chen, Meihua Huang, Jian Cao, Meiling Cao, Zhihui Wang, Guiping Wan, Tao Gui
Bioengineered · 2022 · vol. 13(5) , pp. 12637–12649 · doi:10.1080/21655979.2022.2077614 · PMID:35603555 · W4281260390
article OA: gold CC0 ⤵ 6 in-corpus citations
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RNA-sequencing of adenomyotic endometrial stromal cells identified differentially expressed genes involved in cell-cell adhesion and cytokine-cytokine receptor interactions, highlighting IL-6 and EGF as central hub genes.

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Abstract

This study aimed to identify differentially expressed genes (DEGs) and molecular pathways in eutopic endometrial stromal cells (EuESCs) from adenomyosis (AM) patients and to provide a new insight into the disease mechanisms. The gene expression profiles in adenomyotic EuESCs (A-EuESCs) and normal ESCs (N-ESCs) were analyzed by RNA-sequencing (RNA-Seq) and validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analyses were performed to obtain insights into the functions of DEGs. The protein-protein interaction (PPI) network was constructed using the STRING database and visualized by Cytoscape software, and their hub genes were identified. A total of 458 up-/363 down-regulated genes were identified in A-EuESCs versus N-ESCs. The GO enrichment analysis showed that these genes were significantly enriched in calcium-dependent cell-cell adhesion. The most significant term of the KEGG pathway analysis was cytokine-cytokine receptor interaction. There were 145 nodes in the PPI network of the 157 DEGs, which were identified in significant enrichment pathway by the KEGG pathway analysis in N-ESCs and A-EuESCs. The PPI network revealed that IL-6 was a central hub gene. Besides, IL-6 was found as a central hub gene in the pro-inflammatory/chemotactic subnetwork, and EGF was noted as a central hub gene in the angiogenesis subnetwork. Our study indicated the alterations of transcriptomic profiles in A-EuESCs and provided new insights into the pathogenesis of AM. The A-EuESCs in women with AM have fundamental abnormalities that may predispose to pro-invasion/migration and angiogenesis.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Computational Biology Computational Biology Computational Biology Computational Biology Computational Biology Computational Biology Computational Biology Computational Biology Computational Biology

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