Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve

David B. Seifer; D B Seifer; C Charland; Colette Charland; David L. Berlinsky; D Berlinsky; Alan S. Penzias; A S Penzias; Ray V. Haning; R V Haning; F Naftolin; Frederick Naftolin; B E Barker; Barbara E. Barker

doi:10.1016/0002-9378(93)90430-q

Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve

David B. Seifer, D B Seifer, C Charland, Colette Charland, David L. Berlinsky, D Berlinsky, Alan S. Penzias, A S Penzias, Ray V. Haning, R V Haning, F Naftolin, Frederick Naftolin, B E Barker, Barbara E. Barker

American journal of obstetrics and gynecology · 1993 · vol. 169(6) , pp. 1531–1535 · doi:10.1016/0002-9378(93)90430-q · PMID:8267057 · W1985122025

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This study investigated the proliferative index of human luteinized granulosa cells and found it varies significantly depending on the patient's ovarian reserve.

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Abstract

OBJECTIVE: We examined whether the proliferative index of luteinized granulosa cells, as determined by flow cytometry, varied as a function of a woman's ovarian reserve, as reserve, as reflected by follicular-phase day 3 serum follicle-stimulating hormone. STUDY DESIGN: This prospective cohort study consisted of 19 women of similar chronologic age preparing for in vitro fertilization-embryo who met specific day 3 serum follicle-stimulating hormone criteria. The "low follicle-stimulating hormone" group consisted of 11 women with day 3 serum follicle-stimulating hormone levels or = 18 IU/L. A total of 56 preovulatory follicles containing > or = 10(4) luteinized granulosa cells were examined by flow cytometry. The low follicle-stimulating hormone group was compared with the high follicle-stimulating hormone group to examine proliferative index as a function of serum day 3 follicle-stimulating hormone levels. RESULTS: The low follicle-stimulating hormone group had a greater proliferative index (11.1% +/- 0.4%) than did the high follicle-stimulating hormone group (8.3% +/- 0.6%), p or = 18 IU/L have a 25% decreased proliferative index compared with luteinized granulosa cells from women with day 3 serum follicle-stimulating hormone levels < or = 6. CONCLUSIONS: This suggests that granulosa cell proliferation is influenced by ovarian reserve and may explain in part the more favorable response to ovulation induction protocols that younger women demonstrate compared with women of more advanced reproductive age.

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MeSH descriptors

Follicle Stimulating Hormone Follicular Phase Granulosa Cells Adult Aging Aging Cell Division Female Flow Cytometry Follicle Stimulating Hormone Follicular Phase Granulosa Cells Humans Linear Models Prospective Studies

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Cites (3)

Cited by (1)

Influence of age on results of infertility treatments 1996

References (23)

Analysis of 1071 gift procedures--the case for a flexible approach to treatment via openalex
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Cited by (1)

Influence of age on results of infertility treatments 1996

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[1] Ovulation induction in women age 40 and older: the importance of basal follicle-stimulating hormone level and chronological age 1992

[2] Analysis of 1071 gift procedures--the case for a flexible approach to treatment 1988

[3] An analysis of factors influencing the establishment of a clinical pregnancy in an ultrasound-based ambulatory in vitro fertilization program 1988