PENGARUH PEMBERIAN GENISTEIN TERHADAP PENURUNAN KADAR TNF ALPHA PADA ZALIR PERITONEAL MENCIT MODEL ENDOMETRIOSIS

Rr. Catur Leny Wulandari
In: EMBRIO · 2018 · vol. 10(1) , pp. 1–12 · doi:10.36456/embrio.vol10.no1.a1429 · W2902695139
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Genistein administration significantly reduced TNF-α levels in the peritoneal fluid of mice with endometriosis, with the lowest levels observed at a dose of 100 mg/day.

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Penelitian eksperimental true experimental dengan randomized post-test control group design ini menilai pengaruh pemberian genistein pada kadar TNF-α dalam cairan peritoneal mencit (Mus musculus) dengan model endometriosis. Hewan dibagi menjadi delapan kelompok (kontrol negatif, kontrol positif, serta genistein dosis 50–500 mg/hari), dan kadar TNF-α diukur menggunakan ELISA. Hasilnya menunjukkan adanya pengaruh genistein terhadap penurunan TNF-α (p<0,05), dengan dosis 100 mg/hari sebagai yang terendah (148.25±7.89 pg/ml). Paper ini menegaskan bahwa penurunan TNF-α diamati pada tingkat biomarker dan dilakukan pada model hewan, sehingga generalisasi ke kondisi klinis tidak dibahas secara eksplisit. This paper is centrally about endometriosis — specifically evaluating genistein’s effect on TNF-α levels in peritoneal fluid in a mouse endometriosis model.

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian genistein terhadap penurunan kadar TNF-α studi pada (Mus musculus) model endometriosis. Penelitian dibagi menjadi 8 kelompok, yaitu : kontrol negatif, kontrol positif dengan perlakuan pemberian genistein dosis 50 mg/hari, 100 mg/hari, 200 mg/hari, 300 mg/hari, 400 mg/hari, 500 mg/hari. Pengukuran kadar TNF-α menggunakan ELISA kit. Data hasil pengamatan dianalisis dengan uji Kruskal Wallis dan uji Perbandingan berganda |Ri-Rj| 5%.Menggunakan mencit model endometriosis.Penelitian ini merupakan penelitian eksperimental murni (true experimental) dengan menggunakan rancangan percobaan dengan metode randomized post-test control group design., dilakukan di Laboratorium Fisiologi Reproduksi Embriologi Fakultas Kedokteran Hewan Universitas Airlangga Surabaya dan Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya. Ada pengaruh pemberian genistein terhadap kadar TNF-α dalam cairan peritoneal mencit model endometriosis (p<0,05). Genistein mampu menurunkan kadar TNF-α terendah pada dosis 100 mg/hari (148.25±7.89 pg/ml) bila dibandingkan dengan perlakuan dosis yang lain.
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PENGARUH PEMBERIAN GENISTEIN TERHADAP PENURUNAN KADAR TNF ALPHA PADA ZALIR PERITONEAL MENCIT MODEL ENDOMETRIOSIS DOI: https://doi.org/10.36456/embrio.vol10.no1.a1429Keywords: endometriosis, genistein, TNF-α, model endometriosisAbstract Penelitian ini bertujuan untuk mengetahui pengaruh pemberian genistein terhadap penurunan kadar TNF-α studi pada (Mus musculus) model endometriosis. Penelitian dibagi menjadi 8 kelompok, yaitu : kontrol negatif, kontrol positif dengan perlakuan pemberian genistein dosis 50 mg/hari, 100 mg/hari, 200 mg/hari, 300 mg/hari, 400 mg/hari, 500 mg/hari. Pengukuran kadar TNF-α menggunakan ELISA kit. Data hasil pengamatan dianalisis dengan uji Kruskal Wallis dan uji Perbandingan berganda |Ri-Rj| 5%.Menggunakan mencit model endometriosis.Penelitian ini merupakan penelitian eksperimental murni (true experimental) dengan menggunakan rancangan percobaan dengan metode randomized post-test control group design., dilakukan di Laboratorium Fisiologi Reproduksi Embriologi Fakultas Kedokteran Hewan Universitas Airlangga Surabaya dan Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya. Ada pengaruh pemberian genistein terhadap kadar TNF-α dalam cairan peritoneal mencit model endometriosis (p<0,05). Genistein mampu menurunkan kadar TNF-α terendah pada dosis 100 mg/hari (148.25±7.89 pg/ml) bila dibandingkan dengan perlakuan dosis yang lain.References Anwar, M., Baziad, A., dan Prabowo, P. 2011. Endometriosis. Ilmu Kandungan. Ed 3 Cetakan 1. PT Bina Pustaka Sarwono Prawirodihardjo. Jakarta. 239-250 Amberkar, M.V., Meena, K.K., Mor,V., Semwal, A., and Adiga, S. 2010. 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Merchant, K., Kumi-Diaka, J., Rathinavelu, A., Esiobu, N., Zoeller, R., and. Hormann.V. 2012. Genistein Modulation of Immune-Associated Genes in LNCaP Prostate Cancer Cell Line. The Open Prostate Cancer Journal. 5 : 1-7. Meresman, G.F., Bilotas, M.A., Lombardi, E., Tesone, M.,Sueldo,C., and Baranao,R.I. 2003. Effect of GnRH analogues on apoptosis and release of interleukin-1b and vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. Human Reproduction. 18 (9) : 1767-1771. Pilsakova, L., Riecansky, I., and Jagla, F. 2010 The Physiological Actions of Isoflavone Phytoestrogens. Physiological Research. 59 : 651-664. Rahman, S., Islam, R., Swaraz, A.M., Ansari, A., Parvez, A.K., and Paul, D.K. 2012. An insight on genistein as potential pharmacological and therapeutic agent. Asian Pacific Journal of Tropical Biomedicine : S1924-S1937. Riggs, B.L., and Hartmann, L,D. 2003. Selective Estrogen-Receptor Modulators to Clinical Practice Mechanisms of Action and Application. N Engl J Med. 348 : 618-629. Soares, S.R., Varea, A.M., Hidalgo-Mora, J.J., and Pellicer, A. 2012. Pharmacologic therapies in endometriosis : a systematic review. Fertility and Sterility. 98 (3) : 529-555. Sutrisno, S., Mariyani, M., Herawati I., Rinata, E., Jehanara, J., Yueniwati, Y., Nurdiana, N., Noorhamdani, N., Santoso, S., 2014. The effects of genistein as inflammatory and antiangiogenesis in primary endometriosis cell culture. Asian Pasific Journal of Reproduction. 3 (4) : 1-6. Sutrisno, Soehartono, Arsana. 2010. Efek Genistein terhadap Ekpresi eNOS, BCL2 dan Apoptosis pada kultur sel endotel umbilikus (HUVECs) yang mengalami stres oksidatif. Laboratorium Obstetri dan Ginekologi FK UNAIR Surabaya. Winarsi, H. 2005. Isoflavon. Ed 1, Gajah Mada University Press. Yogyakarta. Yavuz, E., Oktem, M., Esinler, I., and Toru, S.A. 2007. 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