Lactobacillus gasseri OLL2809 inhibits development of ectopic endometrial cell in peritoneal cavity via activation of NK cells in a murine endometriosis model

Hiroyuki Itoh, Ito H, Toshihiro Sashihara, Akira Hosono, Shuichi Kaminogawa, Masayuki Uchida
Cytotechnology · 2011 · vol. 63(2) , pp. 205–210 · doi:10.1007/s10616-011-9343-z · PMID:21409454 · W2002796281
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Oral administration of heat-killed Lactobacillus gasseri OLL2809 suppressed endometriosis development in mice by enhancing NK cell activity through increased IL-2 and NKp46 gene expression.

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This paper tested whether oral administration of heat-killed Lactobacillus gasseri OLL2809, a probiotic lactobacillus known to stimulate IL-12 production, suppresses development of ectopic endometriotic lesions in a murine endometriosis model. Mice received L. gasseri OLL2809 for 21 consecutive days, resulting in a reduction of ectopic endometriotic lesion development to an extent similar to prior IL-12 effects, alongside increased transcription of IL-2 and NK cell triggering receptor 1 in peritoneal cells and evidence consistent with enhanced splenic NK cell cytotoxicity and NK-cell activation. The authors did not report in the provided text any direct NK-cell depletion or other specificity experiments to rule out non-NK pathways. Relevance to endometriosis: the study directly evaluates L. gasseri OLL2809 as an inhibitor of ectopic endometrial lesion development via activation of NK cells in a murine endometriosis model.

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Abstract

We have previously reported that peritoneal administration of interleukin-12 (IL-12) suppresses development of ectopic endometriotic lesions via activation of natural killer (NK) cells in a mouse endometriosis model. Lactobacillus gasseri OLL2809 is one of a probiotic lactobacillus that has been selected on an ability to stimulate production of IL-12 from murine splenocytes. In this study, we examined whether the oral administration of heat-killed L. gasseri OLL2809 suppressed development of endometriosis. Administration of L. gasseri OLL2809 for 21 consecutive days resulted in reduction of the development of ectopic endometriotic lesions in an extent similar to IL-12. Although obvious effects of L. gasseri OLL2809 on the peritoneal cytokine levels, population of peritoneal cells as well as cytotoxicity of splenic NK cells, gene expression analysis of the peritoneal cells revealed enhancement in the transcription of IL-2 and natural killer cell triggering receptor 1 genes. Therefore, it was suggested that L. gasseri OLL2809 suppressed development of endometriosis via activation of NK cells. Similar content being viewed by others Abbreviations - IL: - Interleukin - IFN: - Interferon - NK: - Natural killer - Th: - Helper T cells

References

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