Expression and activation of mitogen-activated protein kinase in the human endometrium during the menstrual cycle

Tomoya Ozaki, Kentaro Takahashi, Haruhiko Kanasaki, Kohji Miyazaki
American journal of obstetrics and gynecology · 2006 · vol. 195(5) , pp. 1343–1350 · doi:10.1016/j.ajog.2006.05.014 · PMID:16796978 · W2025291316
Other OA: closed CC0 ⤵ 2 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-13 ⓘ

This study investigated the expression and activation of mitogen-activated protein kinase (MAPK) within the human endometrium throughout the menstrual cycle.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

OBJECTIVE: The purpose of this study was to investigate the fluctuation of expression and activation of mitogen-activated protein kinase in normal human endometrium throughout the menstrual cycle. STUDY DESIGN: Thirty-three normal endometrial tissues were obtained from fertile women who had undergone hysterectomies for reasons other than endometrial disease. Extracellular signal-regulated kinase, -1, and -2 expression were studied by immunohistochemistry. Moreover, extracellular signal-regulated kinase activity was analyzed by gel kinase assay. RESULTS: Western blotting analysis with anti-pan-extracellular signal-regulated kinase antibody mainly demonstrated an immunoreactive band of 42 kd that corresponded to extracellular signal-regulated kinase 2 in the endometrium. The expression of extracellular signal-regulated kinase 2 tended to increase in the secretory phase. Immunohistochemical analysis for extracellular signal-regulated kinase 1 in endometrial sections revealed a weak staining of glands and almost no staining of stromal cells. Immunohistochemical analysis for extracellular signal-regulated kinase 2 in endometrial sections revealed a distinct staining of glands in both proliferative and secretory phases and a weak staining of stromal cells. Although the intensity of staining for extracellular signal-regulated kinase 2 in stromal cells did not change during the secretory phase, in the glands the extracellular signal-regulated kinase 2 was highly stained in the mid-to-late secretory phase. In gel kinase assay revealed that extracellular signal-regulated kinase activity was increased significantly in the mid-to-late secretory phase. CONCLUSION: Expression and activation of extracellular signal-regulated kinase in the human endometrium was increased particularly during the secretory phase. We suggest that fluctuation of extracellular signal-regulated kinase in the human endometrium may be induced by ovarian steroid hormones.

My notes (saved in your browser only)

MeSH descriptors

Endometrium Menstrual Cycle Mitogen-Activated Protein Kinases Adult Endometrium Enzyme Activation Female Humans Immunohistochemistry Immunohistochemistry Isoenzymes Isoenzymes Luteal Phase Luteal Phase Menstrual Cycle Middle Aged Mitogen-Activated Protein Kinases Staining and Labeling

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cited by (2)

References (35)

Cited by (2)

Source provenance

openalex
last seen: 2026-05-11T05:42:58.953575+00:00
pubmed
last seen: 2026-10-08T21:03:51.313027+00:00
unpaywall
last seen: 2026-10-19T06:34:38.229974+00:00
License: CC0 · commercial use OK