Diagnostic Accuracy of Plasma MicroRNA 125b-5p and 141-3p for Early Endometriosis

Enrollment

60

Start

2023-04-15

Completion

2025-10-15

Last updated

2026-05-14

Has results

False

Countries

Egypt

Conditions

Tagged with

Interventions

• Staging Laparoscopy PROCEDURE

  This is the surgical "gold standard" used for definitive diagnosis. Performed under intravenous anesthesia, the procedure involves the visual detection and description of EM lesions (size, appearance, and depth). Findings are staged from I-IV according to the revised American Society for Reproductive Medicine (ASRM) guidelines.

• Laboratory Biomarker Assessment (Mirs and CA-125) DIAGNOSTIC_TEST

  Two blood samples are drawn from each patient following their menstrual cycle. One sample is used to measure serum carbohydrate antigen (CA)-125 levels via ELISA. The second sample is used to estimate the plasma expression levels (fold change) of Mir-141-3p and Mir-125b-5p using quantitative real-time PCR (rt-PCR).

• Transvaginal Ultrasound (TVS) Staging DIAGNOSTIC_TEST

  Patients undergo pelvic imaging using a 7.5-MHz transvaginal probe. The procedure follows a standardized 5-domain US-based approach to identify specific endometriosis phenotypes and grade the severity of lesions prior to surgery.