Change profiles in matrix metalloproteinase-2 and -9 in induced endometriosis in mice

Summary To examine the changes in matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in the development and progression of endometriosis, real time quantitative polymerase chain reaction, enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21st day after the induction in mice with induced endometriosis (experimental group) and sham-operated animals (controls). The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group. MMP-9 had two or three peaks during the 21 days, taking place at day 1, 4 and 15. It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis. Similar content being viewed by others References Chen QH, Qu JY, Xu YY, et al. Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in ectopic and eutopic endometrium. Chin J Obstet Gynecol (Chinese), 2004,39(12):809–812 Li T, Li YG, Pu DM. Matrix metalloproteinase-2 and -9 expression correlated with angiogenesis in human adenomyosis. Gynecol Obstet Invest 2006, 62: 229–35. Vincent Lagente, Elisabeth Boichot. Matrix metallo-proteinases in tissue remodelliing and inflammation. In: Gasche Y, Copin JC. Matrix metalloproteinases and inflammatory diseases of the central nervous system. Germany Berlin: Die Deutche Bibliothek, 2008:123–152 Chen QH, Zhou WD, Pu DM, et al. The inhibitory effect of 15-R-LXA4 on experimental endometriosis. EJOG, 2009,145(2):200–204 Cao ZY. Chinese Gynecology and Obstetrics. In: Gao Y. Pathology of Ectopic Endometrium. Beijing: People’s Medical Publishing House, 2004,1486–1488 Lang JH. Researches and hypostheses on ectopic endometrium. Chin J Obstet Gynecol (Chinese), 2003, 38(8):478–480 Sternlicht M, Werb Z. How matrix metalloproteinases regulate cell behavior. Annu Rev Cell Dev Biol, 2001,17: 463–516 Gilabert-Estellés J, Ramón LA, España F, et al. Expression of angiogenic factors in endometriosis: relationship to fibrinolytic and metalloproteinase systems. Hum Reprod, 2007,22(8):2120–2127 Chen QH, Zhou WD, Su ZY, et al. Change of pro-inflammatory cytokines follows certain patterns after induction of endometriosis in a mouse model. Fertil Steril, 2010,93(5):1448–1454 Parks WC, Wilson C, Lopez-Boado YS. Matrix metalloproteinases as modulators of inflammation and innate immunity. Nat Rev Immunol, 2004,4(8):617–629 Mulayim N, Savlu A, Guzeloglu-Kayisli O, et al. Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8. Fertil Steril, 2004,81(Suppl 1):904–911 Man YC, Liu Y, Xie W, et al. Effect of blockage of Wnt/β-catenin signaling pathway on activation eutopic endometrial stromal cell of patients with endometriosis by 17β-estradiol treatment. Acta Med Univ Sci Technol Huazhong (Chinese), 2009,38(4):421–424,429 Mott JD, Werb Z. Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol, 2004,16(5): 558–564 Author information Authors and Affiliations Corresponding author Additional information This project was supported by grants from Xiamen Municipal Scientific Research Program (No. 3502 Z 20077047), fund for Medical Research Projects of Scientific and Technological Program (No. 3502 Z 20084006) and a medical innovation-supporting program of Fujian Province (No. 2009-CXB-49). Rights and permissions About this article Cite this article Chen, Q., Qiu, N., Pu, D. et al. Change profiles in matrix metalloproteinase-2 and -9 in induced endometriosis in mice. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 30, 188–192 (2010). https://doi.org/10.1007/s11596-010-0211-4 Received: Published: Issue date: DOI: https://doi.org/10.1007/s11596-010-0211-4