Inhibition of human placental progesterone synthesis by danazol in vitro

T. Rabe; L Kiesel; Ludwig Kiesel; B. Runnebaum

doi:10.1016/s0015-0282(16)47295-x

Inhibition of human placental progesterone synthesis by danazol in vitro

T. Rabe, L Kiesel, Ludwig Kiesel, B. Runnebaum

Fertility and sterility · 1983 · vol. 40(3) , pp. 330–333 · doi:10.1016/s0015-0282(16)47295-x · PMID:6576913 · W2419185577

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Danazol was found to inhibit progesterone synthesis in human placental tissue samples incubated in vitro.

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Abstract

In vitro, danazol showed a slight dose-dependent inhibition of the mitochondrial cholesterol side chain cleavage enzyme isolated from early gestational (8th to 12th week of gestation) placenta. In the presence of 100 microM danazol, the enzyme activity was 65% of controls. Danazol inhibits dose-dependently the mitochondrial 3 beta-hydroxysteroid dehydrogenase (I50 = 3.1 microM; Ki = 1 microM) (noncompetitive inhibition) and the cytoplasmic 20 alpha-hydroxysteroid dehydrogenase (I50 = 1.4 microM; Ki = 2.6 microM) (competitive inhibition). The inhibition of human placental progesterone synthesis by danazol in vitro is a further example for the direct interference of danazol with steroidogenesis.

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MeSH descriptors

Danazol Placenta Pregnadienes Progesterone 20-Hydroxysteroid Dehydrogenases 20-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Danazol Dose-Response Relationship, Drug Female Humans In Vitro Techniques Mitochondria Mitochondria Placenta Placenta Pregnadienes Pregnancy Pregnancy Trimester, First

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References (12)

Danazol Inhibits Steroidogenesis via openalex
Danazol suppresses luteal function in vitro and in vivo via openalex
W1986096348 via openalex
doi:10.1016/s0039-128x(81)90381-0 via openalex
doi:10.1016/s0015-0282(16)47125-6 via openalex
W2468237684 via openalex
W6657442222 via openalex
doi:10.1097/00006254-197802000-00001 via openalex
doi:10.1016/0022-4731(82)90029-2 via openalex
doi:10.1016/0002-9378(81)90611-6 via openalex
doi:10.1016/0022-4731(82)90637-9 via openalex
doi:10.1021/bi00700a031 via openalex

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[1] Danazol Inhibits Steroidogenesis 1977

[2] Danazol suppresses luteal function in vitro and in vivo 1980