Relationship between hMLHl Expression-Deficiency and It's Promoter Methylation in Endometriosis

Li‐Li Shan; A. R. B Y. J. T. Yue; Rong Zhuo; Xiao Tianhui

doi:10.3877/cma.j.issn.1673-5250.2011.03.005

Relationship between hMLHl Expression-Deficiency and It's Promoter Methylation in Endometriosis

Li‐Li Shan, A. R. B Y. J. T. Yue, Rong Zhuo, Xiao Tianhui

In: Chung-Hua Fu Ch'an K'o Tsa Chih · 2011 · vol. 7(3) , pp. 206–209 · doi:10.3877/cma.j.issn.1673-5250.2011.03.005 · W3031689370

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Endometriosis tissues exhibited significantly lower hMLH1 expression and increased promoter methylation compared to normal endometrium.

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This study investigated the expression of the mismatch repair gene hMLH1 and its promoter methylation status in ovarian endometriosis tissue compared to normal endometrium. Using immunohistochemistry and methylation-specific PCR on specimens from 23 patients with endometriosis and 20 controls, researchers found significantly lower hMLH1 expression and higher promoter methylation rates in the endometriosis group. The authors concluded that hMLH1 deficiency plays a key role in the pathogenesis of endometriosis, with promoter methylation identified as a partial mechanism driving this loss of expression. This paper is centrally about endometriosis — specifically examining the molecular mechanisms involving DNA mismatch repair genes in ovarian endometriotic lesions.

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Abstract

Objective To study the methylation of human mutL homolog 1 (hMLH1) promoter and its expression in endometriosis (EMs) and normal endometrium. Methods The protein expression of hMLH1 was analysed in 23 tissues of endometriosis (EMs group) and 20 tissues of normal endometrium (control group) by the immunohistochemistry SP method from January to October 2009. DNA was extracted from all these tissues. Methylation of hMLH1 was verified by methylation specificity PCR(MSP). Results The positive expression of hMLH1 in EMs group was 65% (15/23), control group was 95 % (19/ 20). There was significant difference between two groups (P<0.05). hMLH1 promoter complete and partial methylation in endometriosis was 39% (9/23), control group was 5% (1/20). There was also significant difference between two groups (P<0.05). Conclusion The negative or low expression of hMLH1 was one of the most important mechanisms of endometriosis. Methylation of hMLH1 promoter was partial mechanisms. Key words: endometriosis ( EMs); mismatch repair gene ( MMR); human mutL homolog 1 (hMLH1); immunohistochemistry; methylation

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Objective

To study the methylation of human mutL homolog 1 (hMLH1) promoter and its expression in endometriosis (EMs) and normal endometrium.

Methods

The protein expression of hMLH1 was analysed in 23 tissues of endometriosis (EMs group) and 20 tissues of normal endometrium (control group) by the immunohistochemistry SP method from January to October 2009. DNA was extracted from all these tissues. Methylation of hMLH1 was verified by methylation specificity PCR(MSP).

Results

The positive expression of hMLH1 in EMs group was 65% (15/23), control group was 95 % (19/ 20). There was significant difference between two groups (P<0.05). hMLH1 promoter complete and partial methylation in endometriosis was 39% (9/23), control group was 5% (1/20). There was also significant difference between two groups (P<0.05).

Conclusion

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