Roles of cell migration and invasion mediated by Twist in endometriosis

Juanqing Li, Junyan Ma, Xiangwei Fei, Tao Zhang, Jianhong Zhou, Jun Lin
Journal of Obstetrics and Gynaecology Research · 2019 · vol. 45(8) , pp. 1488–1496 · doi:10.1111/jog.14001 · PMID:31250947 · W2955696008
article OA: closed CC0 ⤵ 27 in-corpus citations
View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

This study found that Twist protein and mRNA expression were highest in endometriosis ectopic endometrium, and Twist overexpression promoted endometrial stromal cell migration and invasion, likely via epithelial-to-mesenchymal transition.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

AIM: To investigate the roles of cell migration and invasion mediated by Twist in endometriosis. METHODS: The protein levels and locations of Twist, N-cadherin and E-cadherin were measured by Western blot and immunohistochemistry in ectopic endometrium and eutopic endometrium of ovarian endometriosis as well as normal endometrium of nonendometriosis patients. The messenger RNA (mRNA) expressions of Twist, N-cadherin and E-cadherin in these tissues were measured by quantitative reverse transcription polymerase chain reaction. Stable overexpression of Twist in eutopic endometrial stromal cells was transfected with a plasmid-mediated delivery system. The protein and mRNA expressions of N-cadherin and E-cadherin were detected by western blot and reverse transcription polymerase chain reaction. The changes of migration and invasion of endometrial stromal cells were explored by transwell. RESULTS: Levels of protein and mRNA of Twist and N-cadherin showed the highest expression in ectopic endometrium of ovarian endometriosis, while lowest in normal endometrium of nonendometriosis patients. On the contrary, the expression of E-cadherin showed highest in normal endometrium of nonendometriosis patients. The overexpression of Twist after transfection significantly upregulated the protein and mRNA expression of N-cadherin, while downregulated the protein and mRNA expression of E-cadherin. There is significant difference between groups. For transwell, the overexpression of Twist in eutopic endometrial stromal cell significantly promoted cell migration and invasion. CONCLUSION: Twist might be related with the increase of migration and invasion in endometrial stromal cells, mediated by epithelial-to-mesenchymal transition.

My notes (saved in your browser only)

Condition tags

mesh:D004715endometriosis

MeSH descriptors

Antigens, CD Cadherins Endometriosis Endometrium Epithelial-Mesenchymal Transition Nuclear Proteins Ovarian Diseases Twist-Related Protein 1 Adult Antigens, CD Cadherins Cell Movement Endometriosis Endometrium Endometrium Female Humans Nuclear Proteins Ovarian Diseases RNA, Messenger

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (28)

Cited by (27)

Source provenance

europepmc
last seen: 2026-06-04T01:30:01.192114+00:00
openalex
last seen: 2026-06-04T00:00:01.174412+00:00
pubmed
last seen: 2026-05-13T22:22:41.077124+00:00
License: CC0 · commercial use OK