The extracellular signal-regulated kinase 1/2 triggers angiogenesis in human ectopic endometrial implants by inducing angioblast differentiation and proliferation

Sefa Arlier, Sefa Arlıer, William Murk, Murk W, Ozlem Guzeloglu‐Kayisli, Guzeloglu-Kayisli O, Ozlem Guzeloglu-Kayisli, Semerci N, Nihan Semerci, Kellie Larsen, Larsen K, Mehmet S Tabak, Tabak MS, Mehmet Selcuk Tabak, Aydın Arıcı, Arici A, Aydin Arici, Frederick Schatz, Schatz F, Lockwood CJ, Charles J. Lockwood, Umit A. Kayisli
American journal of reproductive immunology (New York, N.Y. : 1989) · 2017 · vol. 78(6) · doi:10.1111/aji.12760 · PMID:28921734 · W2755066066
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Extracellular signal-regulated kinase 1/2 activation in human endometrial endothelial cells promotes ectopic endometrial angiogenesis by inducing angioblast differentiation and proliferation.

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Abstract

PROBLEM: The role of extracellular signal-regulated kinase (ERK)1/2-mediated angiogenesis during endometriotic nidation is unknown. We posit that ERK1/2-induced angioblast differentiation and proliferation promotes ectopic endometrial angiogenesis. METHODS OF STUDY: Human eutopic and ectopic endometria were immunostained for total- (T-) or phosphorylated- (P-) ERK1/2 or double-immunostained for P-ERK1/2-CD34 and PCNA-CD34. Estradiol (E2 ), cytokines, normal peritoneal fluid (NPF) or endometriotic peritoneal fluid (EPF) ±PD98059, an ERK1/2 inhibitor, treaded primary human endometrial endothelial cells (HEECs) were evaluated by T-/P-ERK1/2 immunoblotting, MTT viability and tube formation assays. RESULTS: HEECs exhibited higher endothelial P-ERK1/2 immunoreactivity in ectopic vs eutopic endometria. Double-immunostained ectopic endometria displayed abundant CD34-positive angioblasts exhibiting strong P-ERK1/2 and PCNA immunoreactivity. EPF and vascular growth factor (VEGF)-A significantly increased HEEC proliferation and P-ERK1/2 levels. PD98059 reduced basal, EPF, and VEGF-induced HEEC proliferation and promoted vascular stabilization following tube formation. CONCLUSION: Enhanced ERK1/2 activity in angioblasts by such peritoneal factors as VEGF, E2 induces proliferation to trigger ectopic endometrial angiogenesis.

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Condition tags

endometriosis

MeSH descriptors

Choristoma Endometriosis Endometrium Endothelial Cells Extracellular Signal-Regulated MAP Kinases Neovascularization, Pathologic Neovascularization, Pathologic Cell Differentiation Cell Differentiation Cell Proliferation Cell Proliferation Cells, Cultured Cells, Cultured Choristoma Choristoma Endometriosis Endometriosis Endometrium Endometrium Endothelial Cells

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