Altered VEGF, Bcl-2 and IDH1 expression in patients with adenomyosis

Cem Yalaza, Necmiye Canacankatan, İclal Gürses, Hakan Aytan, Bahar Taşdelen
Archives of gynecology and obstetrics · 2020 · vol. 302(5) , pp. 1221–1227 · doi:10.1007/s00404-020-05742-9 · PMID:32785780 · W3048604449
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This study investigated angiogenic, apoptotic, and energy metabolism markers in adenomyosis, finding increased VEGF and Bcl-2 in ectopic endometrium and decreased IDH1 in ectopic and eutopic endometrium compared to controls.

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Abstract

Purpose Adenomyosis is a benign uterine disease resulting from the myometrial invasion of the endometrial gland and stroma. In the current study, angiogenesis, apoptosis and energy metabolism were investigated in adenomyosis.

Methods

A retrospective study was performed using paraffin archival tissues. Three groups were included in the study: Group I and Group II; ectopic and eutopic endometrial tissues of patients with adenomyosis, respectively, and Control Group; endometrial tissue of individuals without adenomyosis. Vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), intercellular adhesion molecule 1 (ICAM-1) and hypoxia-inducible factor 1 alpha (HIF-1A) levels were evaluated as angiogenic markers. Bcl-2, caspase-9 and caspase-3 levels were investigated as apoptotic indicators, and isocitrate dehydrogenase 1 (IDH1), succinate dehydrogenase complex subunit C (SDHC) and fumarate hydratase (FH) levels were also examined as energy metabolism markers. Gene expression levels of all parameters were determined by RT-PCR.

Result

VEGF expression levels were found to be increased in Group I according to the control group and Group II. Bcl-2 expression levels were found to be increased in the Group I compared to the Group II. It was determined that expression levels of IDH1 were decreased in the Group I and Group II compared to the Control Group. There was no significant difference in the other examined parameters. Although we did not find a significant difference in HIF-1A levels between the groups, we found a positive correlation between VEGF and HIF-1A in the Group I.

Conclusion

These results point out that VEGF, HIF-1A, Bcl-2 and IDH1 may be associated with the etiology of adenomyosis. Similar content being viewed by others

References

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Acknowledgements

This study, which is the part of ‘‘Investigation of Angiogenesis, Apoptosis and Energy Metabolism in Adenomyosis’’ project, was funded by grants from Mersin University Research Foundation, Mersin, Turkey. (Grant no. 2017-2-TP3-2580) Funding This study was supported by the Mersin University Research Foundation in Turkey with Project Number 2017-2-TP3-2580. Author information Authors and Affiliations Contributions CY: Project development, Data collection, Molecular analysis, Data analysis, Manuscript writing and editing. NC: Project advisor, Manuscript editing. İG: Data collection, Metodology. HA: Data collection, Clinic advisor. BT: Statistical analysis. Corresponding author Ethics declarations Conflict of interest No authors have any financial/conflicting interests to disclose. Ethical approval This study, which is the part of ‘‘Investigation of Angiogenesis, Apoptosis and Energy Metabolism in Adenomyosis’’ project, was approved by the Ethics Committee of Mersin University (Mersin, Turkey) (13/04/2017, 93). Additional information Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Rights and permissions About this article Cite this article Yalaza, C., Canacankatan, N., Gürses, İ. et al. Altered VEGF, Bcl-2 and IDH1 expression in patients with adenomyosis. Arch Gynecol Obstet 302, 1221–1227 (2020). https://doi.org/10.1007/s00404-020-05742-9 Received: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s00404-020-05742-9

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adenomyosis

MeSH descriptors

Adenomyosis Isocitrate Dehydrogenase Proto-Oncogene Proteins c-bcl-2 Vascular Endothelial Growth Factors Adenomyosis Adult Case-Control Studies Caspase 3 Endometrium Endometrium Female Humans Hypoxia-Inducible Factor 1, alpha Subunit Hypoxia-Inducible Factor 1, alpha Subunit Isocitrate Dehydrogenase Middle Aged Neovascularization, Pathologic Proto-Oncogene Proteins c-bcl-2 Retrospective Studies Vascular Endothelial Growth Factors

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