Exogenous Human β Defensin 2 Protein Regulate Proliferation/apoptosis and Expression of Inflammatory Factor in Endometriosis Cells

【Objective】This study was designed to discuss exogenous human β defensin 2 protein how to regulate the proliferation/apoptosis and the expression of inflammatory factor in endometriosis cells in vitro.【Methods】The endometriosis cells were cultured in vitro,the expression of TNF-α and IL-1β protein in endometriosis cells with/without 40 μg/mL hβD-2 protein was detected by Western blot analysis;when the endometriosis cells were cultured with setting concentration gradient(80 μg/mL,40 μg/mL,20 μg/mL,and 10 μg/mL) hβD-2 protein,on the concentration of TNF-α and IL-1β in the endometriosis cells conditioned medium was detected by ELISA;the endometriosis cells were cultured with adding high-dose hβD-2(120 μg/mL) protein 24H as experimental group,the control group was endometriosis cells cultured with complete medium,the proliferation of two groups were detected by MTT.Cell apoptosis in the two groups were dyed by PI and detected by flow cytometry.【Results】The endometriosis cells expressed hβD-2,TNF-α,and IL-1β.The expression of TNF-α and IL-1β protein in the endometriosis cells can be down-regulated by exogenous hβD-2 protein;the expression of TNF-α and IL-1β protein in endometriosis cells conditioned medium were down-regulated also by exogenous hβD-2 protein,and with dose dependence,the difference have statistical significance(80 μg/mL40 μg/mL20 μg/mL10 μg/mL,P 0.05).Compared with control group,high concentration of hβD-2 protein(120 μg/mL) significantly inhibited cell proliferation and promoted apoptosis(P0.05).【Conclusion】 hβD-2 can influence on expression of TNF-α and IL-1β inflammatory factors in the endometriosis cells,and which can inhibit the proliferation and promote the apoptosis of the endometriosis cells,all of that can provide new theoretical basis for treating endometriosis in the future.