Peripheral blood subpopulations of Bregs producing IL‐35 in women with endometriosis

Anna Sławek, Daria Lorek, A Kedzierska, Paulina Kubik, Jaroslaw Pajak, J Pajak, Agnieszka Chrobak, Anna Chełmońska‐Soyta, Anna Chelmonska-Soyta
American journal of reproductive immunology (New York, N.Y. : 1989) · 2022 · vol. 89(3) , pp. e13675 · doi:10.1111/aji.13675 · PMID:36579639 · W4313280002
article OA: hybrid CC0 ⤵ 3 in-corpus citations
🔓 Open OA copy View on OpenAlex View on PubMed View at publisher

Abstract

PROBLEM: Interleukin 35 (IL-35) is involved in the pathogenesis of endometriosis by suppressing immunoreaction and promoting endometrial cell proliferation. It may also be an essential cytokine in forming the immunosuppressive functions of regulatory B lymphocytes (Bregs). The involvement of Bregs in the pathogenesis of endometriosis has not been previously investigated. In this study, we determined the frequencies of different Breg subpopulations, namely, B10, immature B-cells, and plasmablasts, and their abilities to produce IL-35 in women with endometriosis compared to healthy women. METHODS: The frequencies of different subpopulations of Bregs producing IL-35 were measured in the peripheral blood of women with endometriosis (total pool), women with deep infiltration endometriosis (DIE), women with ovarian endometriosis, and healthy women as a control by flow cytometry. RESULTS: We observed a decrease in the percentage of B10 cells and plasmablasts in women with endometriosis and an increase in the percentage of these Breg populations producing IL-35 in the same experimental group. Interestingly, we also revealed that women with DIE had increased percentages of B10 cells and plasmablasts producing IL-35. CONCLUSION: Taken together, our findings are the first to reveal the frequencies of different subpopulations of Bregs producing IL-35 in women with endometriosis. The results suggest that IL-35 expression in B lymphocytes could be used as a peripheral marker of endometriosis; however, further studies are needed.

My notes (saved in your browser only)

Condition tags

mesh:D004715endometriosisdie_deep_infiltrating

MeSH descriptors

B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory B-Lymphocytes, Regulatory Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (39)

Cited by (3)

Source provenance

europepmc
last seen: 2026-06-04T01:30:01.192114+00:00
openalex
last seen: 2026-06-04T00:00:01.174412+00:00
pubmed
last seen: 2026-06-02T00:34:31.346735+00:00
License: CC0 · commercial use OK