Association of miR-10a expression and its rs3809783 polymorphism with postoperative incisional pain via Bcl-6/Tfh targeting in patients undergoing laparoscopic cholecystectomy

Sun Y, Zhao L, Zhang Z, Li H, Fu X
American journal of clinical and experimental immunology · 2026 · doi:10.62347/qnrh4270 · PMID:42787617 · PMC13601837
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This paper investigated the association of miR-10a expression and its rs3809783 polymorphism with postoperative incisional pain via Bcl-6/Tfh targeting in patients undergoing laparoscopic cholecystectomy.

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This study investigated the role of miR-10a and its rs3809783 polymorphism in postoperative incisional pain among patients undergoing laparoscopic cholecystectomy for acute cholecystitis. The researchers measured miR-10a levels and immune markers in gallstone mucosal tissue, finding that lower miR-10a expression was negatively correlated with pain intensity and associated with the AT genotype, which enhances Bcl-6-mediated Tfh polarization. Mechanistic exploration using a mouse model confirmed Bcl-6 as a direct target of miR-10a, linking specific genetic variants to increased inflammatory markers and complications. Relevance to endometriosis: listed as one indication for GnRH antagonists, though the paper's main focus is uterine fibroids.

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Abstract

This study aimed to investigate the role of miR-10a and its rs3809783 polymorphism in postoperative incisional pain among patients undergoing laparoscopic cholecystectomy for acute cholecystitis (ACC-LC). Levels of miR-10a and immune markers (CD68, CD19, CD3, T-bet, GATA-3, IL-17A, Bcl-6) were measured in gallstone mucosal tissue, and genotyping was performed via PCR and sequencing. An incisional pain mouse model was used for mechanistic exploration. The results showed that miR-10a expression was decreased in ACC-LC patients compared with controls and was negatively correlated with pain intensity. Patients carrying the rs3809783 AT genotype exhibited lower miR-10a levels and higher pain scores than AA carriers. Among the elevated immune markers, only Bcl-6/Tfh was associated with pain severity. Further analysis confirmed Bcl-6 as a direct target of miR-10a, with the A→T substitution at rs3809783 enhancing Bcl-6-mediated Tfh polarization. This genotype was also correlated with longer anal exhaust time, higher inflammatory markers, and more complications. In conclusion, miR-10a and its rs3809783 polymorphism may serve as biomarkers for postoperative pain in ACC-LC by regulating the Bcl-6/Tfh pathway.
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Abstract

This study aimed to investigate the role of miR-10a and its rs3809783 polymorphism in postoperative incisional pain among patients undergoing laparoscopic cholecystectomy for acute cholecystitis (ACC-LC). Levels of miR-10a and immune markers (CD68, CD19, CD3, T-bet, GATA-3, IL-17A, Bcl-6) were measured in gallstone mucosal tissue, and genotyping was performed via PCR and sequencing. An incisional pain mouse model was used for mechanistic exploration. The results showed that miR-10a expression was decreased in ACC-LC patients compared with controls and was negatively correlated with pain intensity. Patients carrying the rs3809783 AT genotype exhibited lower miR-10a levels and higher pain scores than AA carriers. Among the elevated immune markers, only Bcl-6/Tfh was associated with pain severity. Further analysis confirmed Bcl-6 as a direct target of miR-10a, with the A→T substitution at rs3809783 enhancing Bcl-6-mediated Tfh polarization. This genotype was also correlated with longer anal exhaust time, higher inflammatory markers, and more complications. In conclusion, miR-10a and its rs3809783 polymorphism may serve as biomarkers for postoperative pain in ACC-LC by regulating the Bcl-6/Tfh pathway.

Keywords

MicroRNA; T follicular helper cells; laparoscopic cholecystectomy; polymorphism; postoperative incisional pain. AJCEI Copyright © 2026. Conflict of interest statement None. Figures

References

- - Shiihara M, Sudo Y, Matsushita N, Kubota T, Hibi Y, Osugi H, Inoue T. Preoperative difficulty assessment of interval laparoscopic cholecystectomy for gallstones. Surgery. 2024;175:1503–1507. - PubMed - - Taheri P, Moinfar Z, Varpaei HA. Comparison of the stress responses after TAP block and epidural anesthesia in patients undergoing elective laparoscopic cholecystectomy under general anesthesia: randomized clinical trial. Clin J Pain. 2023;39:319–325. - PubMed - - Zhao L, Sun Y, Zhang Z, Li H, Fu X. Expression and genetic polymorphisms of CXCR5 are associated with postoperative incisional pain in patients undergoing laparoscopic cholecystectomy. J Laparoendosc Adv Surg Tech A. 2025;35:798–804. - PubMed LinkOut - more resources Full Text Sources

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