Nucleotide-Binding Oligomerization Domain-Containing Protein 1 Regulates Inflammatory Response in Endometriosis

Wei X, Xin Wei, Wei Xin, Yihua Liu, Liu Y, Li W, Weijia Li, Shao X, Xu Shao
Current protein & peptide science · 2022 · vol. 23(2) , pp. 121–128 · doi:10.2174/1389203723666220322125031 · PMID:35319362 · W4221091195
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This study found that Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is elevated in endometriosis and positively correlates with inflammatory cytokines, with ML130 treatment suppressing cell viability and cytokine production in ectopic endometrial stromal cells.

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This study investigated the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) in the inflammatory response associated with endometriosis. Researchers utilized immunohistochemistry, Western blotting, and qRT-PCR to demonstrate that NOD1 levels and pro-inflammatory cytokines such as IL-6, TNF-α, and MCP-1 are significantly elevated in ectopic endometrial tissue compared to normal controls. Furthermore, inhibition of NOD1 using ML130 suppressed cell viability and reduced the production of these inflammatory cytokines in human endometrial stromal cells exposed to peritoneal fluid. This paper is centrally about endometriosis — specifically examining the molecular mechanisms by which NOD1 regulates inflammation in ectopic endometrial lesions.

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Abstract

BACKGROUND: Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is known to play an important role in innate immunity, while the relationship between NOD1 and inflammatory response in endometriosis remains unknown. The present study aims to investigate the effects of NOD1 on inflammatory response in endometriosis. METHODS: Immunohistochemistry staining, Western blot, and qRT-PCR were conducted to investigate the levels of NOD1 and inflammatory cytokines in the endometriotic lesions. A Spearman's rank correlation analysis was conducted to determine the correlations of NOD1 and inflammatory cytokines (interleukin (IL)-6, tumor necrosis factor (TNF)-α, and monocyte chemoattractant protein (MCP)-1). Human endometrial stromal cells (HESCs) were isolated and incubated with peritoneal fluid with or without ML130. Cell viability was determined by using an MTT assay. RESULTS: A significant elevation in NOD1 and inflammatory cytokine was observed in ectopic endometrium. Interestingly, a positive correlation between NOD1 and inflammatory cytokines was observed. In addition, treatment with ML130 significantly suppressed cell viability and the production of inflammatory cytokines in the 20% peritoneal fluid treated ectopic HESCs. CONCLUSIONS: NOD1 is related to the inflammatory response that is involved in endometriosis.
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Abstract

Background: Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is known to play an important role in innate immunity, while the relationship between NOD1 and inflammatory response in endometriosis remains unknown. The present study aims to investigate the effects of NOD1 on inflammatory response in endometriosis.

Methods

Immunohistochemistry staining, Western blot, and qRT-PCR were conducted to investigate the levels of NOD1 and inflammatory cytokines in the endometriotic lesions. A Spearman’s rank correlation analysis was conducted to determine the correlations of NOD1 and inflammatory cytokines (interleukin (IL)-6, tumor necrosis factor (TNF)-α, and monocyte chemoattractant protein (MCP)-1). Human endometrial stromal cells (HESCs) were isolated and incubated with peritoneal fluid with or without ML130. Cell viability was determined by using an MTT assay.

Results

A significant elevation in NOD1 and inflammatory cytokine was observed in ectopic endometrium. Interestingly, a positive correlation between NOD1 and inflammatory cytokines was observed. In addition, treatment with ML130 significantly suppressed cell viability and the production of inflammatory cytokines in the 20% peritoneal fluid treated ectopic HESCs.

Conclusions

NOD1 is related to the inflammatory response that is involved in endometriosis.

Keywords

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein Nod1 Signaling Adaptor Protein

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