Journal of visualized experiments : JoVE

Endometriosis is a common gynecological disorder characterized by the growth of endometrial-like tissue outside the uterine cavity, leading to chronic inflammation, pelvic pain, and infertility. Reliable mouse models are essential for inves…

Women remain underrepresented in clinical drug development despite their unique biological characteristics and growing disease burden. In China, this disparity is particularly concerning given the rising incidence of gynecologic conditions …

The modified cardiometabolic index (MCMI), an integrated measure of visceral fat and lipid metabolism, has been widely applied in metabolic disease research. However, its relationship with endometriosis has not been thoroughly investigated.…

Endometriosis is a prevalent gynecological disorder associated with chronic low-grade inflammation, yet diagnosis is often delayed because symptoms are non-specific and definitive confirmation typically relies on invasive procedures. Compos…

Endometriosis (EM) is a common chronic gynecological disorder in women of reproductive age, yet its pathogenesis remains unclear, and current therapeutic options are limited by significant side effects. Endometriosis itself increases the ri…

Cervico-vaginal atresia is a rare entity, difficult to diagnose and treat, with a higher rate of complications. It is classified as type 1b mullerian anomaly per ASRM and subclass C4 per ESHRE classification. The increased rate of re-stenos…

Pigs are increasingly used as models of human diseases, largely because their anatomy, physiology, and genetics more closely resemble those of humans. Given its relatively high prevalence and incompletely understood pathogenesis, endometrio…

Endometriosis is frequently associated with infertility and postoperative recurrence, and fertility-sparing surgery may be considered when symptoms, endometriomas, adhesions, or deep infiltrating disease are unlikely to be adequately manage…

Endometriosis (EMs) is a systemic inflammatory disease characterized by the growth of endometrial tissue outside the uterus. Its underlying mechanisms remain unclear, contributing to limitations in diagnosis and treatment. This study evalua…

Chronic pelvic pain (CPP) is a heterogeneous condition in which visceral, musculoskeletal, neuropathic, and pain-processing contributors often overlap. Pelvic floor ultrasound can depict morphology and movement, but its CPP-specific evidenc…

Large benign uterine pathologies affect millions of women globally and impose substantial socioeconomic costs. Although hysterectomy remains the definitive treatment, minimally invasive surgery (MIS) methodologies, including vaginal procedu…

Endometriosis (EMs) is a common gynecological disorder frequently associated with infertility, yet the underlying immune mechanisms remain poorly understood. Sun's Bushen Huayu Formula (BSHYF) has been clinically applied to improve fertilit…

Endometrioma (OMA), a subtype of endometriosis characterized by the formation of endometriotic cysts in the ovaries, affects 17-44% of individuals diagnosed with endometriosis. Women with OMA often experience compromised fertility, yet the …

Endometrial tissue lines the inner cavity of the uterus and is under the cyclical control of estrogen and progesterone. It is a tissue that is composed of luminal and glandular epithelium, a stromal compartment, a vascular network, and a co…

The physiology and pathophysiology of the pancreas are complex. Diseases of the pancreas, such as pancreatitis and pancreatic adenocarcinoma (PDAC) have high morbidity and mortality. Intravital imaging (IVI) is a powerful technique enabling…

The surgical management of endometrioma is still a matter of debate. Cystectomy, which is recognized as the standard technique, seems to be associated with a potential reduction in the ovarian reserve due to the inadvertent removal and ther…

Surgical management of ovarian endometrioma in patients desiring fertility is complicated by the need to balance maximal resection of disease with efforts to spare normal ovarian cortex. Optimization of tubal anatomy is another frequent con…

Preserving female fertility is crucial in a multifunctional healthcare system that takes care of patients' future quality of life. Oocyte cryopreservation is recognized by several international scientific societies as the gold standard for …

Endometriosis is a leading cause of pelvic pain and infertility. It is defined by the presence of endometrial tissue in extrauterine locations. The development of novel therapies and diagnostic tools for endometriosis has been limited due i…

In mares, endometrial cysts are associated with endometriosis and can cause maternal recognition failure or compromise and delay pregnancy diagnoses. Historical treatments were invasive and had adverse effects on the endometrium. Hysterosco…

The hippocampus is a highly organized structure in the brain that is a part of the limbic system and is involved in memory formation and consolidation as well as the manifestation of severe brain disorders, including Alzheimer's disease and…

Here, we describe a protocol for the implementation of a heterologous mouse model in which progression of endometriosis can be assessed in real time through noninvasive monitoring of fluorescence emitted by implanted ectopic human endometri…

Anogenital distance (AGD) is a sexually dimorphic attribute, twice longer in males than in females, and a marker of intrauterine hormonal environment. Interest in AGD measurements is increasing due to mounting evidence on their potential cl…

Decidualization is a progesterone-dependent differentiation process of endometrial stromal cells and is a prerequisite for successful embryo implantation. Although many efforts have been made to reveal the underlying mechanisms of deciduali…

Many efforts have been devoted to establish in vitro cell culture systems. These systems are designed to model a vast number of in vivo processes. Cell culture systems arising from human endometrial samples are no exception. Applications ra…