Craig A. Witz — Endo Lab

lab-animal 2010

Imatinib decreases endometrial stromal cell transmesothial migration and proliferation in the extracellular matrix of modeled peritoneum

Fertility and sterility ·doi:10.1016/j.fertnstert.2010.04.018

OBJECTIVE: To characterize imatinib's effect on endometrial stromal cell (ESC) attachment, proliferation, and invasion in modeled peritoneum. DESIGN: In vitro study. SETTING: Academic medical center. PATIENT(S): Twelve normally cycling w…

2010

Pathogenesis of Endometriosis

·doi:10.4324/9780203319390_chapter_5

lab-animal 2009

Peroxisome-proliferator activator receptor-gamma activation decreases attachment of endometrial cells to peritoneal mesothelial cells in an in vitro model of the early endometriotic lesion

Molecular human reproduction ·doi:10.1093/molehr/gap061

The aim of this study was to investigate whether peroxisome proliferator-activated receptor (PPAR)-gamma activation has an effect on the attachment of endometrial cells to peritoneal mesothelial cells in a well-established in vitro model of…

lab-animal 2008

Activin A increases invasiveness of endometrial cells in an in vitro model of human peritoneum

Molecular human reproduction ·doi:10.1093/molehr/gan016

The aim of this study was to investigate whether activin A has an effect on the attachment and/or invasion of endometrial cells in a modeled peritoneum in vitro. Cultured endometrial stromal cells (ESCs) and endometrial epithelial cells (EE…

lab-animal 2008

A potential role for colony-stimulating factor 1 in the genesis of the early endometriotic lesion

Fertility and sterility ·doi:10.1016/j.fertnstert.2008.09.050

OBJECTIVE: To investigate the role(s) of colony-stimulating factor 1 (CSF-1) on the development of early endometriosis in a murine model by comparing rate of lesion formation in mice [1] homozygous for a CSF-1 mutation versus syngeneic cont…

2008

Imatanib alters expression of endometrial stromal cell signaling genes involved in cell proliferation and metastasis

·doi:10.1016/j.fertnstert.2008.07.244

2008

Imatinib decreases the rate of early endometriotic lesion formation in a murine model

·doi:10.1016/j.fertnstert.2008.07.238

lab-animal 2008

Modeling the early endometriotic lesion: mesothelium-endometrial cell co-culture increases endometrial invasion and alters mesothelial and endometrial gene transcription

Fertility and sterility ·doi:10.1016/j.fertnstert.2007.09.047

OBJECTIVE: To determine the role of peritoneal mesothelial cells (PMCs) in the process of endometrial invasion into the peritoneum and to evaluate gene expression after endometrial-PMC co-culture. DESIGN: In vitro study. SETTING: Universi…

2008

Peritoneal mesothelial cell secretory products increase invasion of endometrial stromal cells in an in vitro model of the early endometriotic lesion

·doi:10.1016/j.fertnstert.2008.07.241

2007

An in vivo model suggests that colony stimulating factor-1 plays a critical role in the genesis of the early endometriotic lesion

·doi:10.1016/j.fertnstert.2007.07.703

2007

Imatinib decreases endometrial stromal cell transmesothelial migration and proliferation in the extracellular matrix of modeled peritoneum

·doi:10.1016/j.fertnstert.2007.07.208

lab-animal 2007

IN VITRO MODELS OF ENDOMETRIOSIS

·doi:10.1093/biolreprod/77.s1.71a

There is mounting evidence that retrograde menstruation and implantation of endometrial fragments are the primary factors responsible for development of the majority of endometriotic lesions. Many crucial questions concerning the initial in…

2007

Menstrual endometrial cells from women with endometriosis demonstrate increased adherence to peritoneal cells and increased expression of CD44 splice variants

·doi:10.1016/j.fertnstert.2007.07.717

lab-animal 2005

A novel in vitro model of the early endometriotic lesion demonstrates that attachment of endometrial cells to mesothelial cells is dependent on the source of endometrial cells

Fertility and sterility ·doi:10.1016/j.fertnstert.2004.10.058

OBJECTIVE: To characterize the source of variability in endometrial stromal cell (ESC) binding to peritoneal mesothelial cells (PMC). DESIGN: In vitro study. SETTING: University medical center. PATIENT(S): Reproductive-age women without …

2005

Colony Stimulating Factor-1 Promotes Endometrial Cell Migration and Proliferation in Two and Three-Dimensional Culture

·doi:10.1016/j.fertnstert.2005.07.295

2005

Modeling endometriosis

·doi:10.1016/j.fertnstert.2005.03.023

2005

Protein Kinase C (PKC)-Dependent Signaling Pathways Promote the Genesis of the Early Endometriotic Lesion

·doi:10.1016/j.fertnstert.2005.07.470

2004

Characterization of a novel three-dimensional model of the early endometriotic lesion

·doi:10.1016/j.fertnstert.2004.07.176

review 2004

Pathogenesis of Endometriosis

·doi:10.3109/9780203319390-12

Endometriosis was described as a disease process over 300 years ago. In the late 17th century it was recognized as peritoneal ‘ulcers’ occurring on the surface of the bladder, intestine, and surface of the uterus.1 In the 18th century physi…

2004

Tumor necrosis factor-alpha (TNF-alpha) induces peritoneal mesothelial (PMC), endometrial epithelial (EEC) and endometrial stromal (ESC) cell expression of genes involved in invasion and metastasis

·doi:10.1016/j.fertnstert.2004.07.434

review 2003

Cell adhesion molecules and endometriosis

Seminars in reproductive medicine ·doi:10.1055/s-2003-41324

The pathogenesis of endometriosis remains poorly defined. The interaction of endometrium with peritoneum is an important aspect of the disease process. Cell adhesion molecules (CAMs) are transmembrane receptors that facilitate intercellular…

review 2003

Pathogenesis of endometriosis--current research

Human fertility (Cambridge, England) ·doi:10.1080/1464770312331368973

Proliferative, secretory and menstrual endometrial cells of both the stroma and epithelium adhere to intact peritoneal mesothelium and mesothelial monolayers. Endometrial attachment to the mesothelium appears to occur rapidly (within 1 h) a…

lab-animal 2003

Time series analysis of transmesothelial invasion by endometrial stromal and epithelial cells using three-dimensional confocal microscopy

Fertility and sterility ·doi:10.1016/s0015-0282(02)04834-3

OBJECTIVE: To evaluate endometrial adhesion and invasion of peritoneal mesothelium. DESIGN: Descriptive study using confocal laser-scanning microscopy. SETTING: University-based laboratory. PATIENT(S): Women undergoing surgery for benign…

lab-animal 2002

An in vitro model to study the pathogenesis of the early endometriosis lesion

Annals of the New York Academy of Sciences ·doi:10.1111/j.1749-6632.2002.tb02790.x

OBJECTIVE: To determine if whole fragments of endometrium can adhere to peritoneum with intact mesothelium. DESIGN: Tissue culture and immunohistochemical study. SETTING: University Medical Center. PATIENTS: Reproductive-age women underg…

lab-animal 2002

Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells

Human Reproduction ·doi:10.1093/humrep/17.11.2832

BACKGROUND: To evaluate adhesion of menstrual endometrium (ME) to intact peritoneal mesothelium. METHODS: Explants of peritoneum were cultured for 1 h with ME (n = 6). Specimens were serially sectioned for haematoxylin and eosin stain and …